Bacterial enzyme biosyntheses inhibition: a tool for ecotoxicity assay

A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection

Bacterial detection plays an important role in protecting public health and safety, and thus, substantial research efforts have been directed at developing bacterial sensing methods that are sensitive, specific, inexpensive, and easy to use. We have recently reported a novel "mix-and-read" assay where a fluorogenic DNAzyme probe was used to detect model bacterium E. coli. In this work, we carri...

Pressure: a novel tool for enzyme-linked immunosorbent assay procedure.

The enzyme-linked immunosorbent assay (ELISA) technique is the backbone of the diagnostic assay for detection of infectious and allergic diseases. Here, we demonstrate a unique ELISA method for the detection of an antigen or antibody, in which ELISA steps are carried out under pressure instead of conventional thermal incubation. Pressure-mediated ELISA (PELISA), carried out in 1 h shows more th...

Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition

Enzyme Inhibition in Microfluidics for Re-engineering Bacterial Synthesis Pathways

Title of Thesis: ENZYME INHIBITION IN MICROFLUIDICS FOR RE-ENGINEERING BACTERIAL SYNTHESIS PATHWAYS Dean Larios Berlin, Masters of Science, 2009 Directed By: Professor Gary W. Rubloff Department of Materials Science and Engineering and the Institute for Systems Research Enzyme-functionalized biological microfluidic (EF-BioMEMS) systems are an emerging class of lab-on-chip devices that manipulat...

Application of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep

Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for   epidemiologic studies. The present study was performed with a commercial human en...